ELISA/assay

Fish Interferon gamma, IFN-gamma ELISA Kit

MBS702530

48-Strip-Wells

605 USD

ELISA Kit

Fish Interferon gamma, IFN-gamma ELISA Kit

Interferon gamma, IFN-gamma

Fish Interferon gamma; IFN-gamma ELISA Kit; Interferon gamma; IFN-

interferon gamma; Interferon gamma; interferon gamma; IFN-gamma; immune interferon; interferon, gamma; Immune interferon

IFN-

IFNG; IFNG; IFG; IFI; IFN-gamma

IFNG_HUMAN

Fish

166

This assay has high sensitivity and excellent specificity for detection of Fish IFN-gamma. No significant cross-reactivity or interference between Fish IFN-gamma and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Detection Range: 20 pg/ml-4000 pg/ml. Sensitivity: 10 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Introduction: Interferon-gamma (IFN-gamma) is a dimerized soluble cytokine that is the only member of the type II class of interferons. This interferon was originally called macrophage-activating factor. The IFN-gamma monomer consists of a core of six alpha-helices and an extended unfolded sequence in the C-terminal region. This is shown in the structural models below. The alpha-helices in the core of the structure are numbered 1 to 6. Full length IFN-gamma is 143 amino acids in length, the models are 126 amino acids in length. Affinity for the glycosaminoglycan heparan sulfate resides solely within the deleted sequence of 17 amino acids. In contrast to interferon-alpha and interferon-beta which can be expressed by all cells, IFN-gamma is secreted by Th1 cells, Tc cells, dendritic cells and NK cells. Also known as immune interferon, IFN-gamma is the only Type II interferon. It is serologically distinct from Type I interferons and it is acid-labile, while the type I variants are acid-stable. IFN-gamma has antiviral, immunoregulatory, and anti-tumour properties. It alters transcription in up to 30 genes producing a variety of physiological and cellular responses. Amongst the effects are: Increase antigen presentation of macrophages. Activate and increase lysosome activity in macrophages. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES. 3 Suppress Th2 cell activity. Cause normal cells to increase expression of class I MHC molecules. Promotes adhesion and binding required for leukocyte migration Promotes NK cell activity. Activation by IFN-gamma is achieved by its interaction with a heterodimeric receptor consisting of IFNGR1 & IFNGR2 (interferon gamma receptors). IFN-gamma binding to the receptor activates the JAK-STAT pathway. In addition, IFN-gamma activates APCs and promotes Th1 differentiation by upregulating the transcription factor T-bet. IFN-gamma is the hallmark cytokine of Th1 cells (whereas Th2 cells produce IL-4 and Th17 cells produce IL-17). NK cells and CD8+ cytotoxic T cells also produce IFN-gamma. IFN-gamma suppresses osteoclast formation by rapidly degrading the RANK adaptor protein TRAF6 in the RANK-RANKL signaling pathway, which otherwise stimulates the production of NFkappaB. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated IFN-gamma and antibody preparation specific for IFN-gamma, and incubated. Then substrate solutions are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES. 4 added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IFN-gamma in the samples is then determined by comparing the O.D. of the samples to the standard curve.

56786138

NP_000610.2

NM_000619.2

P01579

19,348 Da

ATF-2 Transcription Factor Network Pathway (138006); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485)

This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]

Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

48-Strip-Wells

605 USD

96-Strip-Wells

870

5x96-Strip-Wells

2950

10x96-Strip-Wells

5535