Human inducible nitric oxide synthase, iNOS ELISA Kit | MyBioSource MBS702518 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human inducible nitric oxide synthase, iNOS ELISA Kit

catalog :

MBS702518

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702518

products type :

ELISA Kit

products full name :

Human inducible nitric oxide synthase, iNOS ELISA Kit

products short name :

nitric oxide synthase 2, inducible

products name syn :

Human inducible nitric oxide synthase; iNOS ELISA KIT; HEP-NOS; INOS; NOS; NOS2A; NOS; type II; nitric oxide synthase 2A; nitric oxide synthase 2A (inducible; hepatocytes) ; nitric oxide synthase; macrophage; nitric oxide synthase 2; inducible

other names :

nitric oxide synthase, inducible; Nitric oxide synthase, inducible; nitric oxide synthase, inducible; NOS type II; NOS, type II; inducible NOS; hepatocyte NOS; inducible NO synthase; nitric oxide synthase, macrophage; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase 2, inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2

products gene name :

NOS2

other gene names :

NOS2; NOS2; NOS; INOS; NOS2A; HEP-NOS; NOS2A; HEP-NOS; Inducible NOS; iNOS

uniprot entry name :

NOS2_HUMAN

reactivity :

Human

sequence length :

1153

specificity :

This assay has high sensitivity and excellent specificity for detection of Human NOS2. No significant cross-reactivity or interference between Human NOS2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Detection Range: 0.9 IU/ml-60 IU/ml. Sensitivity: 0.225 IU/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to iNOS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for iNOS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of iNOS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

24041029

ncbi acc num :

NP_000616.3

ncbi gb acc num :

NM_000625.4

uniprot acc num :

P35228

ncbi mol weight :

131,117 Da

ncbi pathways :

AGE/RAGE Pathway (698754); ATF-2 Transcription Factor Network Pathway (138006); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795)

ncbi summary :

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. Cofactor: Heme group . Enzyme regulation: Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage