ELISA/assay

Human alpha-actinin-4, ACTN-4 ELISA Kit

MBS702507

96 Strip Wells

850 USD

ELISA Kit

Human alpha-actinin-4, ACTN-4 ELISA Kit

actinin, alpha 4

Human alpha-actinin-4 (ACTN-4) ELISA Kit; ACTININ-4; DKFZp686K23158; FSGS; FSGS1; actinin alpha4 isoform; actinin; alpha 4

alpha-actinin-4; Alpha-actinin-4; alpha-actinin-4; actinin alpha4 isoform; non-muscle alpha-actinin 4; F-actin cross-linking protein; focal segmental glomerulosclerosis 1; actinin, alpha 4; F-actin cross-linking protein; Non-muscle alpha-actinin 4

ACTN4

ACTN4; ACTN4; FSGS; FSGS1; ACTININ-4

ACTN4_HUMAN

Human

911

This assay has high sensitivity and excellent specificity for detection of human ACTN-4. No significant cross-reactivity or interference between human ACTN-4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human alpha-actinin-4 (ACTN-4) concentrations in serum, plasma, tissue homogenates. Detection Range: 31.25 pg/ml-2000 pg/ml.

Sensitivity: The minimum detectable dose of human ACTN-4 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ACTN-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACTN-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTN-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTN-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

12025678

NP_004915.2

NM_004924.4

O43707

104,854 Da

Adherens Junction Pathway 83070!!Adherens Junction Pathway 481!!Amoebiasis Pathway 167324!!Amoebiasis Pathway 167191!!Arrhythmogenic Right Ventricular Cardiomyopathy Pathway 672454!!Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 117293!!Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 116129!!Cell-Cell Communication Pathway 477132!!Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008]

Function: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions.

96 Strip Wells

850 USD