ELISA/assay

Mouse glial fibrillary acidic protein, GFAP ELISA Kit

MBS702506

48-Strip-Wells

565 USD

ELISA Kit

Mouse glial fibrillary acidic protein, GFAP ELISA Kit

glial fibrillary acidic protein

Mouse glial fibrillary acidic protein; GFAP ELISA Kit; FLJ45472; ; glial fibrillary acidic protein

glial fibrillary acidic protein isoform 1; Glial fibrillary acidic protein; glial fibrillary acidic protein; intermediate filament protein; glial fibrillary acidic protein

GFAP

Gfap; Gfap; AI836096; GFAP

GFAP_MOUSE

Mouse

428

This assay has high sensitivity and excellent specificity for detection of mouse GFAP. No significant cross-reactivity or interference between mouse GFAP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml -200 pg/ml. Sensitivity: The minimum detectable dose of mouse GFAP is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GFAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GFAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GFAP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GFAP bound in the initial step. The color development is stopped and the intensity of the color is measured.

196115327

NP_001124492.1

NM_001131020.1

P03995

49,900 Da

Nuclear Signaling By ERBB4 Pathway (1000926); Signal Transduction Pathway (970540); Signaling By ERBB4 Pathway (971124); Spinal Cord Injury Pathway (739004)

Function: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150