Human Pulmonary surfactant-associated protein B, SP-B ELISA Kit | MyBioSource MBS702496 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Pulmonary surfactant-associated protein B, SP-B ELISA Kit

catalog :

MBS702496

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702496

products type :

ELISA Kit

products full name :

Human Pulmonary surfactant-associated protein B, SP-B ELISA Kit

products short name :

surfactant protein B

products name syn :

Human Pulmonary surfactant-associated protein B; SP-B ELISA Kit; PSP-B; SFTB3; SFTP3; SMDP1; SP-B; OTTHUMP00000203253; Pulmonary surfactant-associated protein B; 18kD; surfactant; pulmonary-associated protein B; surfactant protein B

other names :

pulmonary surfactant-associated protein B; Pulmonary surfactant-associated protein B; pulmonary surfactant-associated protein B; 6 kDa protein; 18 kDa pulmonary-surfactant protein; pulmonary surfactant-associated proteolipid SPL(Phe); surfactant protein B; 18 kDa pulmonary-surfactant protein; 6 kDa protein; Pulmonary surfactant-associated proteolipid SPL(Phe)

products gene name :

SFTPB

other gene names :

SFTPB; SFTPB; SP-B; PSP-B; SFTB3; SFTP3; SMDP1; SFTP3; SP-B

uniprot entry name :

PSPB_HUMAN

reactivity :

Human

sequence length :

393

specificity :

This assay has high sensitivity and excellent specificity for detection of human SP-B. No significant cross-reactivity or interference between human SP-B and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, urine and cell culture supernates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human SP-B is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP-B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-B bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

288856299

ncbi acc num :

NP_000533.3

ncbi gb acc num :

NM_000542.3

uniprot acc num :

P07988

ncbi mol weight :

42,117 Da

ncbi pathways :

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

ncbi summary :

This gene encodes the pulmonary-associated surfactant protein B (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, Feb 2010]

uniprot summary :

Function: Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage