catalog number :
MBS702472
products type :
ELISA Kit
products full name :
Rat Interleukin 12, IL-12/P40 ELISA Kit
products short name :
Interleukin 12, IL-12/P40
products name syn :
Rat Interleukin 12; IL-12/P40 ELISA KIT; Interleukin 12; IL-12/P40
other names :
interleukin 12; Interleukin-12 subunit alpha; interleukin-12 subunit alpha; CLMF p35; IL35 subunit; IL-12, subunit p35; interleukin 12, p35; interleukin-12 alpha chain; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1; NKSF1
products gene name :
IL-12/P40
other gene names :
IL12A; IL12A; P35; CLMF; NFSK; NKSF1; IL-12A; NKSF1; IL-12A; CLMF p35; NKSF1
uniprot entry name :
IL12A_HUMAN
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.8 pg/ml
other info2 :
Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
For Samples For the quantitative determination of rat interleukin 12 (IL-12/P40) concentrations in serum, plasma and tissue homogenates. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-12/P40 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12/P40 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-12/P40 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-12/P40 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAD16432.1
ncbi mol weight :
24,874 Da
ncbi pathways :
African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine-cytokine Receptor Interaction Pathway (83051)
ncbi summary :
This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]
uniprot summary :
Function: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
size4 :
10x96-Strip-Wells