catalog number :
MBS702436
products type :
ELISA Kit
products full name :
Rat Pancreatic prohormone, PPY ELISA Kit
products short name :
pancreatic polypeptide
products name syn :
Rat Pancreatic prohormone (PPY) ELISA kit; PNP; pancreatic polypeptide Y; pancreatic polypeptide
other names :
Pancreatic hormone; Pancreatic hormone; Pancreatic polypeptide; PP
other gene names :
PPY; PH; PP
uniprot entry name :
PAHO_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat PPY. No significant cross-reactivity or interference between rat PPY and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 0.78 pg/ml -50 pg/ml. Sensitivity: 0.2 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PPY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPY is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPY bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
4,197 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)
uniprot summary :
Function: Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions.
size4 :
10x96-Strip-Wells