ELISA/assay

Rat interferon-inducible protein 10, IP-10 ELISA Kit

MBS702428

48-Strip-Wells

565 USD

ELISA Kit

Rat interferon-inducible protein 10, IP-10 ELISA Kit

chemokine (C-X-C motif) ligand 10

Rat interferon-inducible protein 10; IP-10 ELISA Kit; C7; IFI10; INP10; IP-10; SCYB10; crg-2; gIP-10; mob-1; gamma IP10; interferon-inducible cytokine IP-10; protein 10 from interferon (gamma) -induced cell line; small inducible cytokine B10; small inducibl; chemokine (C-X-C motif) ligand 10

C-X-C motif chemokine 10; C-X-C motif chemokine 10; C-X-C motif chemokine 10; gamma-IP10; protein Mob-1; small-inducible cytokine B10; interferon-inducible protein 10; interferon-inducible cytokine IP-10; 10 kDa interferon gamma-induced protein; small inducible cytokine B subfamily (Cys-X-Cys) member 10; small inducible cytokine B subfamily (Cys-X-Cys), member 10; chemokine (C-X-C motif) ligand 10; 10 kDa interferon gamma-induced protein; Gamma-IP10; IP-10; Interferon-inducible protein 10; Protein Mob-1; Small-inducible cytokine B10

CXCL10

Cxcl10; Cxcl10; IP-10; Scyb10; Inp10; Mob1; Scyb10; Gamma-IP10; IP-10

CXL10_RAT

Rat

98

This assay has high sensitivity and excellent specificity for detection of rat IP-10. No significant cross-reactivity or interference between rat IP-10 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 781.25 pg/ml -50000 pg/ml. Sensitivity: 195 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IP-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IP-10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IP-10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IP-10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

20806125

NP_620789.1

NM_139089.1

P48973

10,729 Da

Chemokine Receptors Bind Chemokines Pathway (1010166); Chemokine Signaling Pathway (98755); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (1010164); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125115); Cytosolic DNA-sensing Pathway (124833); G Alpha (i) Signalling Events Pathway (1010195); GPCR Downstream Signaling Pathway (1010193)

induces DNA synthesis, cell proliferation, and cell migration in vascular smooth muscle cells; may play a role in vascular remodeling [RGD, Feb 2006]

Function: In addition to its role as a proinflammatory cytokine, may participate in T-cell effector function and perhaps T-cell development

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150