Human Pulmonary surfactant-associated protein A, SP-A ELISA Kit | MyBioSource MBS702421 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Pulmonary surfactant-associated protein A, SP-A ELISA Kit

catalog :

MBS702421

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702421

products type :

ELISA Kit

products full name :

Human Pulmonary surfactant-associated protein A, SP-A ELISA Kit

products short name :

Pulmonary surfactant-associated protein A1

products name syn :

Human Pulmonary surfactant-associated protein A; SP-A ELISA Kit; AC068139.6; MGC133365; PSAP; PSPA; SFTP1; SFTPA1; pulmonary surfactant apoprotein; surfactant; pulmonary-associated protein A1; surfactant; pulmonary-associated protein A1B; Pulmonary surfactant-associated protein A1

other names :

pulmonary surfactant-associated protein A1 isoform 2; Pulmonary surfactant-associated protein A1; pulmonary surfactant-associated protein A1; collectin-4; surfactant protein A1B; alveolar proteinosis protein; surfactant protein A1 variant AD' 6A; surfactant protein A1 variant ACD' 6A; surfactant protein A1 variant AD' 6A2; surfactant protein A1 variant AD' 6A3; surfactant protein A1 variant AD' 6A4; surfactant protein A1 variant AB'D' 6A; surfactant protein A1 variant ACD' 6A2; surfactant protein A1 variant ACD' 6A3; surfactant protein A1 variant ACD' 6A4; surfactant protein A1 variant AB'D' 6A2; surfactant protein A1 variant AB'D' 6A3; surfactant protein A1 variant AB'D' 6A4; surfactant, pulmonary-associated protein A1A; surfactant, pulmonary-associated protein A1B; 35 kDa pulmonary surfactant-associated protein; surfactant protein A1; 35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein; Collectin-4

products gene name :

SFTPA1

other gene names :

SFTPA1; SFTPA1; SPA; PSAP; PSPA; SP-A; SPA1; PSP-A; SFTP1; SP-A1; COLEC4; SFTPA1B; COLEC4; PSAP; SFTP1; SFTPA; SFTPA1B; PSP-A; PSPA; SP-A; SP-A1

uniprot entry name :

SFTA1_HUMAN

reactivity :

Human

sequence length :

263

specificity :

This assay has high sensitivity and excellent specificity for detection of human SP-A. No significant cross-reactivity or interference between human SP-A and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, urine. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of human SP-A is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP-A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP-A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-A bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

257467606

ncbi acc num :

NP_001087239.2

ncbi gb acc num :

NM_001093770.2

uniprot acc num :

Q8IWL2

ncbi mol weight :

26,242 Da

ncbi pathways :

FOXA1 Transcription Factor Network Pathway (137979); Pertussis Pathway (218111); Pertussis Pathway (218099); Phagosome Pathway (153910); Phagosome Pathway (153859)

ncbi summary :

This gene encodes a lung surfactant protein that is a member of a subfamily of C-type lectins called collectins. The encoded protein binds specific carbohydrate moieties found on lipids and on the surface of microorganisms. This protein plays an essential role in surfactant homeostasis and in the defense against respiratory pathogens. Mutations in this gene are associated with idiopathic pulmonary fibrosis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

uniprot summary :

Function: In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration.

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage