Human soluble Cluster of differentiation 86, sCD86 ELISA Kit | MyBioSource MBS702416 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human soluble Cluster of differentiation 86, sCD86 ELISA Kit

catalog :

MBS702416

quantity :

48-Strip-Wells

price :

370 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702416

products type :

ELISA Kit

products full name :

Human soluble Cluster of differentiation 86, sCD86 ELISA Kit

products short name :

CD86 molecule

products name syn :

Human soluble Cluster of differentiation 86; sCD86 ELISA Kit; B7-2; B70; CD28LG2; LAB72; MGC34413; B-lymphocyte activation antigen B7-2; B7-2 antigen; CD28 antigen ligand 2; CD86 antigen; CD86 antigen (CD28 antigen ligand 2; B7-2 antigen) ; CTLA-4 counter-receptor B; CD86 molecule

other names :

T-lymphocyte activation antigen CD86 isoform 4; T-lymphocyte activation antigen CD86; T-lymphocyte activation antigen CD86; BU63; FUN-1; CTLA-4 counter-receptor B7.2; B-lymphocyte activation antigen B7-2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 molecule; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD_antigen: CD86

products gene name :

CD86

other gene names :

CD86; CD86; B70; B7-2; B7.2; LAB72; CD28LG2; CD28LG2

uniprot entry name :

CD86_HUMAN

reactivity :

Human

sequence length :

217

specificity :

This assay has high sensitivity and excellent specificity for detection of human sCD86. No significant cross-reactivity or interference between human sCD86 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: The minimum detectable dose of human sCD86 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sCD86 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD86 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sCD86 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD86 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

332634950

ncbi acc num :

NP_001193853.1

ncbi gb acc num :

NM_001206924.1

uniprot acc num :

P42081

ncbi mol weight :

37,682 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); CD28 Co-stimulation Pathway (119553); CD28 Dependent PI3K/Akt Signaling Pathway (119554); CD28 Dependent Vav1 Pathway (119555); CTLA4 Inhibitory Signaling Pathway (119556)

ncbi summary :

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

uniprot summary :

Function: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.

size1 :

48-Strip-Wells

price1 :

370 USD

size2 :

96-Strip-Wells

price2 :

525

size3 :

5x96-Strip-Wells

price3 :

2025

size4 :

10x96-Strip-Wells

price4 :

3775

more info or order :

MyBioSource product webpage