catalog number :
MBS702407
products type :
ELISA Kit
products full name :
Mouse Neuron-specific enolase, NSE ELISA Kit
products short name :
enolase 2 (gamma, neuronal)
products name syn :
Mouse Neuron-specific enolase; NSE ELISA Kit; NSE; 2-phospho-D-glycerate hydrolyase; enolase 2; neural enolase; neuron specific gamma enolase; neurone-specific enolase; enolase 2 (gamma; neuronal)
other names :
gamma-enolase; Gamma-enolase; gamma-enolase; neural enolase; neuron-specific enolase; 2-phospho-D-glycerate hydro-lyase; enolase 2, gamma neuronal; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE
products gene name :
ENO2
other gene names :
Eno2; Eno2; NSE; Eno-2; AI837106; D6Ertd375e; Eno-2; NSE
uniprot entry name :
ENOG_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse ENO2. No significant cross-reactivity or interference between Mouse ENO2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml-100 ng/ml. Sensitivity: 0.39 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: glyconeogenesis and glycometabolism. Protein Biological Process 3: Glycolysis
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NSE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NSE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NSE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NSE bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_038537.1
ncbi gb acc num :
NM_013509.2
ncbi mol weight :
47,297 Da
ncbi pathways :
Biosynthesis Of Amino Acids Pathway (790952); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (815838); Carbon Metabolism Pathway (817567); Disease Pathway (970606); Gluconeogenesis Pathway (970554); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (421742); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196); Glucose Metabolism Pathway (970557); Glycogen Storage Diseases Pathway (970607)
uniprot summary :
Function: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival UniRule annotationHAMAP-Rule:MF_00318. Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotationHAMAP-Rule:MF_00318. Cofactor: Magnesium. Required for catalysis and for stabilizing the dimer.
size4 :
10x96-Strip-Wells