Human Angiotensin 1-7, Ang1-7 ELISA Kit | MyBioSource MBS702392 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Angiotensin 1-7, Ang1-7 ELISA Kit

catalog :

MBS702392

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

product information

catalog number :

MBS702392

products type :

ELISA Kit

products full name :

Human Angiotensin 1-7, Ang1-7 ELISA Kit

products short name :

[Angiotensin 1-7 (Ang1-7)]

products name syn :

[Human Angiotensin 1-7 (Ang1-7) ELISA Kit; Angiotensin 1-7 (Ang1-7]

other names :

[angiopoietin-1 isoform 2; Angiopoietin-1; angiopoietin-1; ANG-1; angiopoietin 1]

products gene name :

[Ang1-7]

other gene names :

[ANGPT1; ANGPT1; AGP1; AGPT; ANG1; KIAA0003; ANG-1]

uniprot entry name :

ANGP1_HUMAN

reactivity :

Human

sequence length :

497

specificity :

This assay has high sensitivity and excellent specificity for detection of human Ang1-7. No significant cross-reactivity or interference between human Ang1-7 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, cell culture supernates. Assay Type: Quantitative Sandwich. Detection Range: 7.8 pg/ml - 500 pg/ml. Sensitivity: Typically less than 1.95 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Ang1-7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Ang1-7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Ang1-7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Ang1-7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

315434240

ncbi acc num :

NP_001186788.1

ncbi gb acc num :

NM_001199859.1

uniprot acc num :

Q15389

ncbi mol weight :

57,456 Da

ncbi pathways :

Angiogenesis Pathway (198772); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Cell Surface Interactions At The Vascular Wall Pathway (106062); HIF-1 Signaling Pathway (695200); Hemostasis Pathway (106028); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Rap1 Signaling Pathway (868086); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (868085)

ncbi summary :

Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2010]

uniprot summary :

Function: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.4 PublicationsManual assertion based on experiment in:Ref.10

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145