catalog number :
MBS702388
products type :
ELISA Kit
products full name :
Dog adrenomedullin, ADM ELISA Kit
products short name :
adrenomedullin
products name syn :
Dog adrenomedullin; ADM ELISA Kit; AM; preproadrenomedullin; adrenomedullin
other names :
ADM; ADM; ADM; ProAM N-terminal 20 peptide; PAMP; ProAM-N20
other gene names :
ADM; ADM; AM; PAMP; ProAM-N20
uniprot entry name :
ADML_CANFA
specificity :
This assay has high sensitivity and excellent specificity for detection of Dog ADM. No significant cross-reactivity or interference between Dog ADM and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for adrenomedullin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any adrenomedullin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for adrenomedullin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of adrenomedullin bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001003183.1
ncbi gb acc num :
NM_001003183.1
ncbi mol weight :
20,929 Da
ncbi pathways :
Calcitonin-like Ligand Receptors Pathway (1017215); Class B/2 (Secretin Family Receptors) Pathway (1017214); G Alpha (s) Signalling Events Pathway (1017219); GPCR Downstream Signaling Pathway (1017218); GPCR Ligand Binding Pathway (1017188); Signal Transduction Pathway (1017095); Signaling By GPCR Pathway (1017187)
uniprot summary :
Function: AM and PAMP are potent hypotensive and vasodilatator agents.
size4 :
10x96-Strip-Wells