catalog number :
MBS702387
products type :
ELISA Kit
products full name :
Human Receptor II for the Fc region of immunoglobulin G, FcgammaRII ELISA Kit
products short name :
Receptor II for the Fc region of immunoglobulin G, FcgammaRII
products name syn :
Human Receptor II for the Fc region of immunoglobulin G; FcgammaRII ELISA Kit; Receptor II for the Fc region of immunoglobulin G; FcgammaR
other names :
Fc gamma receptor RII, partial; Fc gamma receptor RII; low affinity immunoglobulin gamma Fc region receptor II-a; Fc gamma receptor RII
products gene name :
FcgammaRII/CD32
other gene names :
FCGR2A; CD32
uniprot entry name :
F5A874_MACMU
specificity :
This assay has high sensitivity and excellent specificity for detection of Human FcgammaRII/CD32. No significant cross-reactivity or interference between Human FcgammaRII/CD32 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 1.56 ng/ml-100 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.39 ng/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FcgammaRII has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FcgammaRII present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FcgammaRII is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FcgammaRII bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AEC03706.1
ncbi mol weight :
31,269 Da
ncbi pathways :
Fc Gamma R-mediated Phagocytosis Pathway 114362!!Fc Gamma R-mediated Phagocytosis Pathway 106590!!Leishmaniasis Pathway 144185!!Leishmaniasis Pathway 144028!!Osteoclast Differentiation Pathway 193151!!Osteoclast Differentiation Pathway 193096!!Phagosome Pathway 153919!!Phagosome Pathway 153859!!Platelet Activation Pathway 953411!!Staphylococcus Aureus Infection Pathway 172860