ELISA/assay

Mouse Nerve growth factor, NGF ELISA Kit

MBS702384

48-Strip-Wells

465 USD

ELISA Kit

Mouse Nerve growth factor, NGF ELISA Kit

nerve growth factor (beta polypeptide)

Mouse Nerve growth factor; NGF ELISA kit; Beta-NGF; HSAN5; MGC161426; MGC161428; NGFB; beta-nerve growth factor; nerve growth factor; beta polypeptide; nerve growth factor; beta subunit; nerve growth factor (beta polypeptide)

beta-nerve growth factor isoform B; Beta-nerve growth factor; beta-nerve growth factor; beta-NGF; nerve growth factor, beta; nerve growth factor

NGF

Ngf; Ngf; Ngfb; Ngfb; Beta-NGF

NGF_MOUSE

Mouse

241

This assay has high sensitivity and excellent specificity for detection of mouse NGF. No significant cross-reactivity or interference between mouse NGF and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 pg/ml -100 pg/ml. Sensitivity: The minimum detectable dose of mouse NGF is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

162951831

NP_001106168.1

NM_001112698.2

P01139

27,077 Da

ARMS-mediated Activation Pathway (970820); Activation Of TRKA Receptors Pathway (1000905); Apoptosis Pathway (83257); Apoptosis Pathway (470); Apoptosis Signaling Pathway (522973); Axonal Growth Stimulation Pathway (1000902); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (1000892); Ceramide Signalling Pathway (1000900); Frs2-mediated Activation Pathway (971392); Id Signaling Pathway (198411)

Function: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival.

48-Strip-Wells

465 USD

96-Strip-Wells

655

5x96-Strip-Wells

2455

10x96-Strip-Wells

4595