Human Kallikrein 7, KLK 7 ELISA Kit | MyBioSource MBS702381 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Kallikrein 7, KLK 7 ELISA Kit

catalog :

MBS702381

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702381

products type :

ELISA Kit

products full name :

Human Kallikrein 7, KLK 7 ELISA Kit

products short name :

kallikrein-related peptidase 7

products name syn :

Human Kallikrein 7; KLK 7 ELISA Kit; PRSS6; SCCE; kallikrein 7 (chymotryptic; stratum corneum) ; protease; serine; 6; signal protein; stratum corneum chymotryptic enzyme; kallikrein-related peptidase 7

other names :

kallikrein-7 isoform 2; Kallikrein-7; kallikrein-7; signal protein; serine protease 6; stratum corneum chymotryptic enzyme; kallikrein 7 (chymotryptic, stratum corneum); kallikrein-related peptidase 7; Serine protease 6; Stratum corneum chymotryptic enzyme; hSCCE

products gene name :

KLK7

other gene names :

KLK7; KLK7; hK7; SCCE; PRSS6; PRSS6; SCCE; hK7; hSCCE

uniprot entry name :

KLK7_HUMAN

reactivity :

Human

sequence length :

181

specificity :

This assay has high sensitivity and excellent specificity for detection of human KLK7. No significant cross-reactivity or interference between human KLK7 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of human KLK7 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KLK 7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLK 7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK 7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK 7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

333609286

ncbi acc num :

NP_001193982.1

ncbi gb acc num :

NM_001207053.1

uniprot acc num :

P49862

ncbi mol weight :

27,525 Da

ncbi pathways :

Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

This gene encodes a member of the kallikrein subfamily of serine proteases. These enzymes have diverse physiological functions and many kallikrein genes are biomarkers for cancer. The encoded protein has chymotrypsin-like activity and plays a role in the proteolysis of intercellular cohesive structures that precedes desquamation, the shedding of the outermost layer of the epidermis. The encoded protein may play a role in cancer invasion and metastasis, and increased expression of this gene is associated with unfavorable prognosis and progression of several types of cancer. Polymorphisms in this gene may play a role in the development of atopic dermatitis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, which is one of fifteen kallikrein subfamily members located in a gene cluster on chromosome 19. [provided by RefSeq, May 2011]

uniprot summary :

Function: May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr- -Gln-15' and insulin B chain at '6-Leu- -Cys-7', '16-Tyr- -Leu-17', '25-Phe- -Tyr-26' and '26-Tyr- -Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.1 PublicationManual assertion based on experiment in:Ref.11. Catalytic activity: Cleavage of proteins with aromatic side chains in the P1 position. Enzyme regulation: Inhibited by Zn2+ and Cu2+ at low micromolar concentrations. Inhibited by SERPINA12.1 PublicationManual assertion based on experiment in:Ref.11

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage