ELISA/assay

Human GlyCated hemoglobin A1c, GHbA1c ELISA Kit

MBS702379

48-Strip-Wells

565 USD

ELISA Kit

Human GlyCated hemoglobin A1c, GHbA1c ELISA Kit

Glycated hemoglobin A1c, GHbA1c

Human Glycated hemoglobin A1c; GHbA1c ELISA Kit; Glycated hemoglobin A1c; GHbA1c

GHbA1c

Human

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Lysates for RBC. Detection Range: 0.78 ng/ml-50ng/ml. Sensitivity: 0.39 ng/ml

Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Introduction: Glycosylated (or glycated) hemoglobin (hemoglobin A1c, Hb1c, or HbA1c, A1C) is a form of hemoglobin used primarily to identify the average plasma glucose concentration over prolonged periods of time. It is formed in a non-enzymatic pathway by hemoglobin's normal exposure to high plasma levels of glucose. Glycosylation of hemoglobin has been implicated in nephropathy and retinopathy in diabetes mellitus. Monitoring the HbA1c in type-1 diabetic patients may improve treatment. In the normal 120-day life span of the red blood cell, glucose molecules join hemoglobin, forming glycated hemoglobin. In individuals with poorly controlled diabetes, increases in the quantities of these glycated hemoglobins are noted. Once a hemoglobin molecule is glycated, it remains that way. A buildup of glycated hemoglobin within the red cell reflects the average level of glucose to which the cell has been exposed during its life cycle. Measuring glycated hemoglobin assesses the effectiveness of therapy by monitoring long-term serum glucose regulation. Higher levels of HbA1c are found in people with persistently elevated blood sugar, as in diabetes mellitus. While diabetic patient treatment goals vary, many include a target range of HbA1c values. A diabetic person with good glucose control has a HbA1c level that is close to or within the reference range. A high HbA1c represents poor glucose control. However, a 'good' HbA1c in a patient with diabetes can still be riddled with a history of recent hypoglycemia, or, alternatively, spikes of hyperglycemia. Regular blood glucose monitoring is still the best method for the analysis of overall vascular health with respect to blood sugar control. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GHbA1c. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for GHbA1c and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GHbA1c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GHbA1c in the samples is then determined by comparing the O.D. of the samples to the standard curve.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150