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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse lipoprotein-associated phospholipase A2, Lp-PLA2 ELISA Kit
catalog :
MBS702378
quantity :
48-Strip-Wells
price :
590 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS702378
products type :
ELISA Kit
products full name :
Mouse lipoprotein-associated phospholipase A2, Lp-PLA2 ELISA Kit
products short name :
phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
products name syn :
Mouse lipoprotein-associated phospholipase A2; Lp-PLA2 ELISA Kit; LDL-PLA2; LP-PLA2; PAFAH; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; PAF 2-acylhydrolase; lipoprotein-associated phospholipase A2; phospholipase A2; ; phospholipase A2; group VII (platelet-activating factor acetylhydrolase; plasma)
other names :
platelet-activating factor acetylhydrolase; Platelet-activating factor acetylhydrolase; platelet-activating factor acetylhydrolase; LDL-PLA(2); PAF 2-acylhydrolase; PAF acetylhydrolase; LDL-associated phospholipase A2; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma); 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; LDL-associated phospholipase A2; LDL-PLA(2); PAF 2-acylhydrolase
products gene name :
PLA2G7
other gene names :
Pla2g7; Pla2g7; R75400; Pafah; PAF acetylhydrolase; LDL-PLA(2)
uniprot entry name :
PAFA_MOUSE
reactivity :
Mouse
sequence length :
440
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse PLA2G7. No significant cross-reactivity or interference between Mouse PLA2G7 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 7.81pg/ml-500 pg/ml. Sensitivity: 1.95 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Lipogenesis and lipometabolism. Protein Biological Process 3: Lipid degradation
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LP-PLA2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for LP-PLA2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain LP-PLA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LP-PLA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi gi num :
31980752
ncbi acc num :
NP_038765.2
ncbi gb acc num :
NM_013737.5
uniprot acc num :
Q60963
ncbi mol weight :
49,258 Da
ncbi pathways :
Ether Lipid Metabolism Pathway (83189); Ether Lipid Metabolism Pathway (365); Metabolic Pathways (132962); Metabolism Of Proteins Pathway (970583); Peptide Hormone Metabolism Pathway (1000413); Synthesis, Secretion, And Deacylation Of Ghrelin Pathway (1000415)
uniprot summary :
Function: Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. Catalytic activity: 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.
size1 :
48-Strip-Wells
price1 :
590 USD
size2 :
96-Strip-Wells
price2 :
850
size3 :
5x96-Strip-Wells
price3 :
2880
size4 :
10x96-Strip-Wells
price4 :
5440
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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