product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human secreted frizzled-related protein 5, SFRP5 ELISA Kit
catalog :
MBS702373
quantity :
48-Strip-Wells
price :
565 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS702373
products type :
ELISA Kit
products full name :
Human secreted frizzled-related protein 5, SFRP5 ELISA Kit
products short name :
secreted frizzled-related protein 5
products name syn :
Human secreted frizzled-related protein 5 (SFRP5) ELISA Kit; SARP3; secreted apoptosis related protein 3; secreted frizzled-related protein 5
other names :
secreted frizzled-related protein 5; Secreted frizzled-related protein 5; secreted frizzled-related protein 5; FRP-1b; SARP-3; sFRP-5; frizzled-related protein 1b; secreted apoptosis related protein 3; secreted apoptosis-related protein 3; secreted frizzled-related protein 5; Frizzled-related protein 1b; FRP-1b; Secreted apoptosis-related protein 3; SARP-3
products gene name :
SFRP5
other gene names :
SFRP5; SFRP5; SARP3; FRP1B; SARP3; sFRP-5; FRP-1b; SARP-3
uniprot entry name :
SFRP5_HUMAN
reactivity :
Human
sequence length :
317
specificity :
This assay has high sensitivity and excellent specificity for detection of human SFRP5. No significant cross-reactivity or interference between human SFRP5 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 pg/ml -100 pg/ml. Sensitivity: The minimum detectable dose of human SFRP5 is typically less than 0.39 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFRP5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFRP5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
188528609
ncbi acc num :
NP_003006.2
ncbi gb acc num :
NM_003015.3
uniprot acc num :
Q5T4F7
ncbi mol weight :
35,563 Da
ncbi pathways :
Wnt Signaling Pathway (83061); Wnt Signaling Pathway (471)
ncbi summary :
Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. [provided by RefSeq, Jul 2008]
uniprot summary :
Function: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina.
size1 :
48-Strip-Wells
price1 :
565 USD
size2 :
96-Strip-Wells
price2 :
810
size3 :
5x96-Strip-Wells
price3 :
2750
size4 :
10x96-Strip-Wells
price4 :
5150
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

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