ELISA/assay

Mouse Pulmonary surfactant-associated protein A, SP-A ELISA Kit

MBS702358

48-Strip-Wells

510 USD

ELISA Kit

Mouse Pulmonary surfactant-associated protein A, SP-A ELISA Kit

surfactant protein A1

Mouse Pulmonary surfactant-associated protein A; SP-A ELISA Kit; AC068139.6; MGC133365; PSAP; PSPA; SFTP1; SFTPA1; pulmonary surfactant apoprotein; surfactant; pulmonary-associated protein A1; surfactant; pulmonary-associated protein A1B; surfactant protein A1

Pulmonary surfactant-associated protein A; Pulmonary surfactant-associated protein A; pulmonary surfactant-associated protein A; PSAP; PSP-A; surfactant pulmonary associated protein A1; surfactant associated protein A1

SFTPA1

Sftpa1; Sftpa1; SP-A; Sftp1; Sftp-1; Sftp-1; Sftp1; Sftpa; PSAP; PSP-A; SP-A

SFTPA_MOUSE

Mouse

248

This assay has high sensitivity and excellent specificity for detection of mouse SP-A. No significant cross-reactivity or interference between mouse SP-A and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml -200 pg/ml. Sensitivity: The minimum detectable dose of mouse SP-A is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP-A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP-A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-A bound in the initial step. The color development is stopped and the intensity of the color is measured.

464483

P35242.1

P35242

26,157 Da

Pertussis Pathway (218112); Pertussis Pathway (218099); Phagosome Pathway (153914); Phagosome Pathway (153859)

Function: In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration.

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800