ELISA/assay

Mouse Caspase 3, Casp-3 ELISA Kit

MBS702352

48-Strip-Wells

465 USD

ELISA Kit

Mouse Caspase 3, Casp-3 ELISA Kit

caspase 3, apoptosis-related cysteine peptidase

Mouse Caspase 3; Casp-3 ELISA Kit; CPP32; CPP32B; SCA-1; PARP cleavage protease; SREBP cleavage activity 1; Yama; apopain; caspase 3; caspase 3; apoptosis-related cysteine protease; cysteine protease CPP32; procaspase3; caspase 3; apoptosis-related cysteine peptidase

caspase-3; Caspase-3; caspase-3; protein Yama; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis related cysteine protease; caspase 3; Apopain; Cysteine protease CPP32; CPP-32; LICE; Protein Yama; SREBP cleavage activity 1; SCA-1

CASP3

Casp3; Casp3; CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Apopain; Caspase-3; A830040C14Rik; Cpp32; CASP-3; CPP-32; SCA-1

CASP3_MOUSE

Mouse

277

This assay has high sensitivity and excellent specificity for detection of mouse Casp-3. No significant cross-reactivity or interference between mouse Casp-3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: 0.078 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Casp-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Casp-3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Casp-3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Casp-3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

549806822

NP_001271338.1

NM_001284409.1

P70677

31,475 Da

Activation Of DNA Fragmentation Factor Pathway (1000455); Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway (1000445); Alpha6-Beta4 Integrin Signaling Pathway (198373); Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Amyotrophic Lateral Sclerosis (ALS) Pathway (83296); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Androgen Receptor Signaling Pathway (198319)

Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Catalytic activity: Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp- - with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.

48-Strip-Wells

465 USD

96-Strip-Wells

655

5x96-Strip-Wells

2455

10x96-Strip-Wells

4595