Human hypoxia-inducible factor 2, HIF-2 ELISA Kit | MyBioSource MBS702348 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human hypoxia-inducible factor 2, HIF-2 ELISA Kit

catalog :

MBS702348

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702348

products type :

ELISA Kit

products full name :

Human hypoxia-inducible factor 2, HIF-2 ELISA Kit

products short name :

endothelial PAS domain protein 1

products name syn :

Human hypoxia-inducible factor 2 (HIF-2) ELISA kit; ECYT4; HIF2A; HLF; MOP2; PASD2; bHLHe73; HIF-1alpha-like factor; basic-helix-loop-helix-PAS protein MOP2; hypoxia-inducible factor 2 alpha; member of PAS protein 2; endothelial PAS domain protein 1

other names :

endothelial PAS domain-containing protein 1; Endothelial PAS domain-containing protein 1; endothelial PAS domain-containing protein 1; EPAS-1; HIF2-alpha; HIF-2-alpha; HIF-1alpha-like factor; HIF-1-alpha-like factor; member of PAS protein 2; PAS domain-containing protein 2; hypoxia-inducible factor 2 alpha; hypoxia-inducible factor 2-alpha; basic-helix-loop-helix-PAS protein MOP2; class E basic helix-loop-helix protein 73; endothelial PAS domain protein 1; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; bHLHe73; HIF-1-alpha-like factor; HLF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Member of PAS protein 2; PAS domain-containing protein 2

products gene name :

EPAS1

other gene names :

EPAS1; EPAS1; HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73; BHLHE73; HIF2A; MOP2; PASD2; EPAS-1; bHLHe73; HLF; HIF-2-alpha; HIF2-alpha

uniprot entry name :

EPAS1_HUMAN

reactivity :

Human

sequence length :

870

specificity :

This assay has high sensitivity and excellent specificity for detection of Human EPAS1. No significant cross-reactivity or interference between Human EPAS1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: 15.6 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Angiogenesis. Protein Biological Process 3: Angiogenesis

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HIF-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIF-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIF-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIF-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

40254439

ncbi acc num :

NP_001421.2

ncbi gb acc num :

NM_001430.4

uniprot acc num :

Q99814

ncbi mol weight :

96,459 Da

ncbi pathways :

Adipogenesis Pathway (198832); Cellular Response To Hypoxia Pathway (645259); Cellular Responses To Stress Pathway (645258); Developmental Biology Pathway (477129); HIF-2-alpha Transcription Factor Network Pathway (137956); Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway (645261); Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway (645262); Pathways In Cancer (83105); Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway (645260); Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway (645263)

ncbi summary :

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]

uniprot summary :

Function: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage