ELISA/assay

Rat coagulation factor XII, FXII ELISA Kit

MBS702347

96 Strip Wells

915 USD

ELISA Kit

Rat coagulation factor XII, FXII ELISA Kit

coagulation factor XII (Hageman factor)

Rat coagulation factor XII; FXII ELISA Kit; HAE3; HAEX; HAF; Hageman factor; beta-factor XIIa part 1; beta-factor XIIa part 2; coagulation factor XII; coagulation factor XIIa heavy chain; coagulation factor XIIa light chain; coagulation factor XII (Hageman factor)

coagulation factor XII; Coagulation factor XII; coagulation factor XII; hageman factor; coagulation factor XII (Hageman factor); Hageman factor; HAF

F12

F12; F12; HAF; HAF

FA12_RAT

Rat

603

This assay has high sensitivity and excellent specificity for detection of rat FXII. No significant cross-reactivity or interference between rat FXII and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of rat coagulation factor XII (FXII) concentrations in serum, plasma, tissue homogenates. Detection Range: 0.625 ng/ml -40 ng/ml.

Sensitivity: The minimum detectable dose of rat FXII is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: </b<This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FXII has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FXII present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FXII is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FXII bound in the initial step. The color development is stopped and the intensity of the color is measured.

62078741

NP_001014028.1

NM_001014006.1

D3ZTE0

65,844 Da

Blood Clotting Cascade Pathway 198493!!Complement And Coagulation Cascades Pathway 83465!!Complement And Coagulation Cascades Pathway 484!!Formation Of Fibrin Clot (Clotting Cascade) Pathway 1010914!!Hemostasis Pathway 1010891!!Intrinsic Pathway 1010916

Function: Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa . Catalytic activity: Selective cleavage of Arg- -Ile bonds in factor VII to form factor VIIa and factor XI to form factor XIa.

96 Strip Wells

915 USD