Dog Vascular Endothelial cell Growth Factor, VEGF ELISA Kit | MyBioSource MBS702337 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Dog Vascular Endothelial cell Growth Factor, VEGF ELISA Kit

catalog :

MBS702337

quantity :

48-Strip-Wells

price :

490 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702337

products type :

ELISA Kit

products full name :

Dog Vascular Endothelial cell Growth Factor, VEGF ELISA Kit

products short name :

vascular endothelial growth factor A

products name syn :

Dog Vascular Endothelial cell Growth Factor; VEGF ELISA KIT; RP1-261G23.1; MGC70609; MVCD1; VEGF; VEGF-A; VPF; vascular endothelial growth factor isoform VEGF165; vascular permeability factor; vascular endothelial growth factor A

other names :

vascular endothelial growth factor A isoform 1; Vascular endothelial growth factor A; vascular endothelial growth factor A; VPF; VEGF-A; vascular permeability factor; vascular endothelial growth factor 188; Vascular permeability factor; VPF

products gene name :

VEGFA

other gene names :

VEGFA; VEGFA; VEGF; VEGF; VEGF-A; VPF

uniprot entry name :

VEGFA_CANFA

reactivity :

Dog

sequence length :

214

specificity :

This assay has high sensitivity and excellent specificity for detection of Dog VEGFA. No significant cross-reactivity or interference between Dog VEGFA and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Detection Range: 3.12 pg/ml-200 pg/ml. Sensitivity: 0.78pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Angiogenesis. Protein Biological Process 3: Angiogenesis

products description :

Introduction: Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) or vasculotropin, is a homodimeric 34 -42 kDa, heparin-binding glycoprotein with potent angiogenic, mitogenic and vascular permeability-enhancing activities specific for endothelial cells. The amino acid sequence of VEGF exhibits primary structural, as well as limited amino acid sequence, homology with that of the A and B chains of PDGF. VEGF is expressed by numerous rodent and tumor cells, including lung adenocarcinoma, bladder carcinoma, fibrosarcoma, HL60 promyelocytic leukemia, GS-9L glioma, and U937 lymphoma cells. In normal tissues, VEGF expression has been found in activated macrophages, keratinocytes, renal glomerular visceral epithelium and mesangial cells, hepatocytes, smooth muscle cells, Leydig cells ,embryonic fibroblasts and bronchial and choroid plexus epithelium. The expression of VEGF is upregulated by phorbol ester, TGF-a and in hypoxia. In the conditioned media of human choriocarcinoma cells (JAR and JE-3), the occurrence of VEGF/PlGF heterodimers has also been observed. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated polyclonal antibody preparation specific for VEGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of VEGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

160358860

ncbi acc num :

NP_001003175.2

ncbi gb acc num :

NM_001003175.2

uniprot acc num :

Q9MYV3

ncbi mol weight :

25,175 Da

ncbi pathways :

Axon Guidance Pathway (1016806); Bladder Cancer Pathway (84161); Bladder Cancer Pathway (527); Cellular Response To Hypoxia Pathway (1016937); Cellular Responses To Stress Pathway (1016936); Cytokine-cytokine Receptor Interaction Pathway (84101); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (1016805); EPH-Ephrin Signaling Pathway (1016833); EPHA-mediated Growth Cone Collapse Pathway (1016834)

uniprot summary :

Function: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin

size1 :

48-Strip-Wells

price1 :

490 USD

size2 :

96-Strip-Wells

price2 :

690

size3 :

5x96-Strip-Wells

price3 :

2560

size4 :

10x96-Strip-Wells

price4 :

4790

more info or order :

MyBioSource product webpage