catalog number :
MBS702320
products type :
ELISA Kit
products full name :
Rat cytochrome P450, CYP450 ELISA Kit
products short name :
cytochrome P450, CYP450
products name syn :
Rat cytochrome P450; CYP450 ELISA Kit; cytochrome P450; CYP450
other names :
cytochrome P450, partial; Cytochrome P450; Cytochrome P450
products gene name :
CYP450
uniprot entry name :
Q6XMT3_CULPI
specificity :
This assay has high sensitivity and excellent specificity for detection of rat CYP1B1. No significant cross-reactivity or interference between rat CYP1B1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of rat CYP1B1 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP1B1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP1B1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP1B1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP1B1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAP57078.1
ncbi mol weight :
17,510 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells