ELISA/assay

Human islet amyloid polypeptide, IAPP ELISA Kit

MBS702315

96 Strip Wells

850 USD

ELISA Kit

Human islet amyloid polypeptide, IAPP ELISA Kit

islet amyloid polypeptide

Human islet amyloid polypeptide; IAPP ELISA Kit; AMYLIN; DAP; IAP; Islet amyloid polypeptide (diabetes-associated peptide; amylin) ; islet amyloid polypeptide

islet amyloid polypeptide; Islet amyloid polypeptide; islet amyloid polypeptide; amylin; insulinoma amyloid peptide; diabetes-associated peptide; Islet amyloid polypeptide (diabetes-associated peptide; amylin); islet amyloid polypeptide; Amylin; Diabetes-associated peptide; DAP; Insulinoma amyloid peptide

IAPP

IAPP; IAPP; DAP; IAP; DAP

IAPP_HUMAN

Human

89

This assay has high sensitivity and excellent specificity for detection of human IAPP. No significant cross-reactivity or interference between human IAPP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human islet amyloid polypeptide?IAPP? concentrations in serum, plasma,urine. Detection Range: 47 pg/ml-3000 pg/ml.

Sensitivity: The minimum detectable dose of human IAPP is typically less than 11.7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IAPP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IAPP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IAPP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IAPP bound in the initial step. The color development is stopped and the intensity of the color is measured.

4557655

NP_000406.1

NM_000415.2

P10997

9,806 Da

Amyloids Pathway 366238!!Calcitonin-like Ligand Receptors Pathway 106379!!Class B/2 (Secretin Family Receptors) Pathway 106378!!Developmental Biology Pathway 477129!!Disease Pathway 530764!!G Alpha (s) Signalling Events Pathway 119549!!GPCR Downstream Signaling Pathway 119548!!GPCR Ligand Binding Pathway 161020!!Integrated Pancreatic Cancer Pathway 711360!!Maturity Onset Diabetes Of The Young Pathway 83096

Islet, or insulinoma, amyloid polypeptide is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring an insulinoma. While the association of amylin with the development of type II diabetes has been known for some time, a direct causative role for amylin has been harder to establish. Studies suggest that amylin, like the related beta-amyloid (Abeta) associated with Alzheimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the development of type II diabetes. [provided by RefSeq, Apr 2011]

Function: Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.

96 Strip Wells

850 USD