catalog number :
MBS702312
products type :
ELISA Kit
products full name :
Human Aquaporin 2, AQP-2 ELISA Kit
products short name :
aquaporin 2 (collecting duct)
products name syn :
Human Aquaporin 2; AQP-2 ELISA Kit; AQP-CD; MGC34501; WCH-CD; aquaporin 2; aquaporin-CD; collecting duct water channel protein; water-channel aquaporin 2; aquaporin 2 (collecting duct)
other names :
aquaporin-2; Aquaporin-2; aquaporin-2; AQP-2; aquaporin-CD; ADH water channel; water-channel aquaporin 2; collecting duct water channel protein; water channel protein for renal collecting duct; aquaporin 2 (collecting duct); ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct
products gene name :
AQP2
other gene names :
AQP2; AQP2; AQP-CD; WCH-CD; AQP-2; AQP-CD
uniprot entry name :
AQP2_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human AQP2. No significant cross-reactivity or interference between Human AQP2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, urine, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 3: Transport
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AQP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AQP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AQP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AQP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_000477.1
ncbi gb acc num :
NM_000486.5
ncbi mol weight :
28,837 Da
ncbi pathways :
Aquaporin-mediated Transport Pathway (187187); Passive Transport By Aquaporins Pathway (187188); Transmembrane Transport Of Small Molecules Pathway (106572); Vasopressin Regulates Renal Water Homeostasis Via Aquaporins Pathway (187189); Vasopressin-regulated Water Reabsorption Pathway (143700); Vasopressin-regulated Water Reabsorption Pathway (143631)
ncbi summary :
This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus. [provided by RefSeq, Oct 2008]
uniprot summary :
Function: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
size4 :
10x96-Strip-Wells