ELISA/assay

Human protein disulfide isomerase, PDI ELISA Kit

MBS702295

96 Strip Wells

850 USD

ELISA Kit

Human protein disulfide isomerase, PDI ELISA Kit

prolyl 4-hydroxylase, beta polypeptide

Human protein disulfide isomerase; PDI ELISA Kit; DSI; ERBA2L; GIT; P4Hbeta; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB; collagen prolyl 4-hydroxylase beta; glutathione-insulin transhydrogenase; procollagen-proline; 2-oxoglutarate 4-dioxygenase (proline 4; prolyl 4-hydroxylase; beta polypeptide

protein disulfide-isomerase; Protein disulfide-isomerase; protein disulfide-isomerase; p55; protocollagen hydroxylase; prolyl 4-hydroxylase subunit beta; collagen prolyl 4-hydroxylase beta; thyroid hormone-binding protein p55; glutathione-insulin transhydrogenase; cellular thyroid hormone-binding protein; protein disulfide isomerase-associated 1; protein disulfide isomerase/oxidoreductase; protein disulfide isomerase family A, member 1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; prolyl 4-hydroxylase, beta polypeptide; Cellular thyroid hormone-binding protein; Prolyl 4-hydroxylase subunit beta; p55

P4HB

P4HB; P4HB; DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; ERBA2L; P4Hbeta; ERBA2L; PDI; PDIA1; PO4DB; PDI

PDIA1_HUMAN

Human

508

This assay has high sensitivity and excellent specificity for detection of human PDI. No significant cross-reactivity or interference between human PDI and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human protein disulfide isomerase (PDI) concentrations in serum, plasma, tissue homogenates. Detection Range: 0.312 ng/ml -20 ng/ml.

Sensitivity: The minimum detectable dose of human PDI is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: </b<This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDI is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDI bound in the initial step. The color development is stopped and the intensity of the color is measured.

20070125

NP_000909.2

NM_000918.3

P07237

57,116 Da

Cellular Responses To Stress Pathway 645258!!Chylomicron-mediated Lipid Transport Pathway 106157!!Collagen Biosynthesis And Modifying Enzymes Pathway 645289!!Collagen Formation Pathway 645288!!Detoxification Of Reactive Oxygen Species Pathway 941400!!Disease Pathway 530764!!Extracellular Matrix Organization Pathway 576262!!Hedgehog Ligand Biogenesis Pathway 1016168!!Lipid Digestion, Mobilization, And Transport Pathway 106111!!Lipoprotein Metabolism Pathway 106156

This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq, Jul 2008]

Function: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.2 PublicationsManual assertion based on experiment in:Ref.17. Catalytic activity: Catalyzes the rearrangement of -S-S- bonds in proteins.

96 Strip Wells

850 USD