product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Vitamin D-binding protein, DBP ELISA Kit
catalog :
MBS702288
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS702288
products type :
ELISA Kit
products full name :
Human Vitamin D-binding protein, DBP ELISA Kit
products short name :
group-specific component (vitamin D binding protein)
products name syn :
Human Vitamin D-binding protein; DBP ELISA Kit; DBP; DBP/GC; GRD3; VDBG; VDBP; vitamin D-binding alpha-globulin; vitamin D-binding protein; group-specific component (vitamin D binding protein)
other names :
vitamin D-binding protein isoform 1; Vitamin D-binding protein; vitamin D-binding protein; VDB; gc-globulin; vitamin D-binding alpha-globulin; epididymis secretory protein Li 51; group-specific component (vitamin D binding protein); Gc-globulin; Group-specific component
products gene name :
GC
other gene names :
GC; GC; DBP; GRD3; VDBG; VDBP; DBP/GC; HEL-S-51; DBP; VDB
uniprot entry name :
VTDB_HUMAN
reactivity :
Human
sequence length :
474
specificity :
This assay has high sensitivity and excellent specificity for detection of human DBP. No significant cross-reactivity or interference between human DBP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 0.156 mug/ml -10 mug/ml. Sensitivity: The minimum detectable dose of human DBP is typically less than 0.039 mug/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DBP. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for DBP. The competitive inhibition reaction is launched between with pre-coated DBP and DBP in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of DBP in the samples. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
32483410
ncbi acc num :
NP_000574.2
ncbi gb acc num :
NM_000583.3
uniprot acc num :
P02774
ncbi mol weight :
52,964 Da
ncbi pathways :
Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamin D Pathway (106150); Vitamin D (calciferol) Metabolism Pathway (160956); Vitamin D Synthesis Pathway (198764)
ncbi summary :
The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]
uniprot summary :
Function: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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