catalog number :
MBS702273
products type :
ELISA Kit
products full name :
Human cellular fibronectin, cFn ELISA Kit
products short name :
cellular fibronectin, cFn
products name syn :
Human cellular fibronectin; cFn ELISA kit; cellular fibronectin; cFn
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Sample Type: serum, plasma, tissue homogenates, cell lysates. Detection Range: 31.25 ng/ml-2000 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 3.12 ng/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to cFn. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for cFn and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain cFn, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of cFn in the samples is then determined by comparing the O.D. of the samples to the standard curve.