ELISA/assay

Mouse connective tissue growth factor, CTGF ELISA Kit

MBS702272

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Mouse connective tissue growth factor, CTGF ELISA Kit

[connective tissue growth factor]

[Mouse connective tissue growth factor; CTGF ELISA Kit; CCN2; HCS24; IGFBP8; MGC102839; NOV2; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8; connective tissue growth factor]

[connective tissue growth factor; Connective tissue growth factor; connective tissue growth factor; CCN family member 2; fibroblast inducible secreted protein; fibroblast inducible secreated protein; hypertrophic chondrocyte-specific protein 24; hypertrophic chondrocyte-specific gene product 24; Connective tissue growth factor precursor (CTGF) (FISP-12 protein) (Hypertrophic chondrocyte-specific protein 24); connective tissue growth factor; CCN family member 2; Hypertrophic chondrocyte-specific protein 24; Protein FISP-12]

[CTGF]

[Ctgf; Ctgf; Ccn2; Hcs24; Fisp12; fisp-12; Ccn2; Fisp-12; Fisp12; Hcs24]

CTGF_MOUSE

Mouse

348

This assay has high sensitivity and excellent specificity for detection of canine CTGF. No significant cross-reactivity or interference between canine CTGF and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: 15.6 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CTGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

171846283

NP_034347.2

NM_010217.2

P29268

37,824 Da

Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (970709); Gene Expression Pathway (971427); Generic Transcription Pathway (1000465); Hippo Signaling Pathway (749786); Hippo Signaling Pathway (750388); Metabolism Pathway (971233); Metabolism Of Lipids And Lipoproteins Pathway (970615); PPARA Activates Gene Expression Pathway (1001047); PodNet: Protein-protein Interactions In The Podocyte Pathway (755428); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (1001046)

Function: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells Enhances fibroblast growth factor-induced DNA synthesis 2 PublicationsManual assertion based on experiment in:Ref.6

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

575

96-Strip-Wells

835

5x96-Strip-Wells

3040

10x96-Strip-Wells

5755