catalog number :
MBS702264
products type :
ELISA Kit
products full name :
Rat brain natriuretic peptide, BNP ELISA Kit
products short name :
brain natriuretic peptide, BNP
products name syn :
Rat brain natriuretic peptide; BNP ELISA Kit; BNP; brain type natriuretic peptide; natriuretic protein; brain natriuretic peptide; BNP
other names :
natriuretic peptides B; Natriuretic peptides B; natriuretic peptides B; iso-ANP; Brain natriuretic factor; brain natriuretic peptide; gamma-brain natriuretic peptide; natriuretic peptide precursor B; natriuretic peptide precursor type B; natriuretic peptide B; Gamma-brain natriuretic peptide; Iso-ANPCleaved into the following chain:Brain natriuretic peptide 45Alternative name(s):5 kDa cardiac natriuretic peptide; BNP-34
other gene names :
Nppb; Nppb; BNP; Bnf
uniprot entry name :
ANFB_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat BNP. No significant cross-reactivity or interference between rat BNP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 125 pg/ml -8000 pg/ml. Sensitivity: The minimum detectable dose of rat BNP is typically less than 31.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BNP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BNP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BNP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BNP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_113733.1
ncbi gb acc num :
NM_031545.1
ncbi mol weight :
13,656 Da
ncbi pathways :
CGMP-PKG Signaling Pathway (983777); CGMP-PKG Signaling Pathway (985584)
ncbi summary :
hormone produced primarily by the atrium and ventricle of the heart [RGD, Feb 2006]
uniprot summary :
Function: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3
size4 :
10x96-Strip-Wells