catalog number :
MBS702259
products type :
ELISA Kit
products full name :
Mouse anti-nuclear Antibody, IgG ELISA Kit
products short name :
anti-nuclear Antibody (IgG)
products name syn :
Mouse anti-nuclear Antibody (IgG) ELISA Kit; anti-nuclear Antibody (IgG)
other names :
ANA; Protein BTG3; protein BTG3; protein Tob5; B-cell translocation gene 3; abundant in neuroepithelium area protein; BTG family, member 3; Abundant in neuroepithelium area protein; BTG family member 3; Protein Tob5
other gene names :
BTG3; BTG3; ANA; TOB5; TOFA; TOB55; ANA; TOB5
uniprot entry name :
BTG3_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse anti-nuclear antibody (IgG). No significant cross-reactivity or interference between mouse anti-nuclear antibody (IgG) and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: The minimum detectable dose of mouse anti-nuclear antibody (IgG) is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: The microtiter plate provided in this kit has been pre-coated with anti-mouse IgG antibody. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antigen and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of anti-nuclear antibody(IgG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
BAA33788.1
ncbi mol weight :
34,163 Da
ncbi pathways :
RNA Degradation Pathway (117291); RNA Degradation Pathway (116127)
ncbi summary :
The protein encoded by this gene is a member of the BTG/Tob family. This family has structurally related proteins that appear to have antiproliferative properties. This encoded protein might play a role in neurogenesis in the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
uniprot summary :
Function: Overexpression impairs serum-induced cell cycle progression from the G0/G1 to S phase.
size4 :
10x96-Strip-Wells