ELISA/assay

Human Dickkopf 1, DKK1 ELISA Kit

MBS702258

96 Strip Wells

670 USD

ELISA Kit

Human Dickkopf 1, DKK1 ELISA Kit

dickkopf homolog 1 (Xenopus laevis)

Human Dickkopf 1; DKK1 ELISA Kit; DKK-1; SK; dickkopf homolog 1; dickkopf related protein-1; dickkopf-1 like; dickkopf homolog 1 (Xenopus laevis)

dickkopf-related protein 1; Dickkopf-related protein 1; dickkopf-related protein 1; hDkk-1; dickkopf-1 like; dickkopf 1 homolog; dickkopf-like protein 1; dickkopf related protein-1; dickkopf WNT signaling pathway inhibitor 1; SK

DKK1

DKK1; DKK1; SK; DKK-1; UNQ492/PRO1008; Dickkopf-1; Dkk-1; hDkk-1

DKK1_HUMAN

Human

266

This assay has high sensitivity and excellent specificity for detection of Human DKK1. No significant cross-reactivity or interference between Human DKK1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 62.5 pg/ml-4000pg/ml. Detection Wavelength: 450 nm. Sensitivity: 15.6pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Signaling Pathway. Protein Biological Process 3: Wnt signaling pathway

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DKK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DKK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DKK1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DKK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

7110719

NP_036374.1

NM_012242.2

O94907

28,672 Da

Cardiac Progenitor Differentiation Pathway 712094!!Direct P53 Effectors Pathway 137939!!Disease Pathway 530764!!Presenilin Action In Notch And Wnt Signaling Pathway 137914!!RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway 1016209!!Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway 169352!!Signal Transduction Pathway 477114!!Signaling By WNT In Cancer Pathway 1016191!!Signaling By Wnt Pathway 106510!!TCF Dependent Signaling In Response To WNT Pathway 980475

This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq, Jul 2008]

Function: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.1 PublicationManual assertion based on experiment in:Ref.15

96 Strip Wells

670 USD