Human Integrin alphaM, ITG/CD11b ELISA Kit | MyBioSource MBS702254 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Integrin alphaM, ITG/CD11b ELISA Kit

catalog :

MBS702254

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702254

products type :

ELISA Kit

products full name :

Human Integrin alphaM, ITG/CD11b ELISA Kit

products short name :

integrin, alpha M (complement component 3 receptor 3 subunit)

products name syn :

Human Integrin alphaM; ITG/CD11b ELISA Kit; CD11B; CR3A; MAC-1; MAC1A; MGC117044; MO1A; SLEB6; antigen CD11b (p170) ; integrin alpha M; macrophage antigen alpha polypeptide; neutrophil adherence receptor alpha-M subunit; integrin; alpha M (complement component 3 receptor 3 subunit)

other names :

integrin alpha-M isoform 2; Integrin alpha-M; integrin alpha-M; CR-3 alpha chain; antigen CD11b (p170); leukocyte adhesion receptor MO1; CD11 antigen-like family member B; macrophage antigen alpha polypeptide; cell surface glycoprotein MAC-1 subunit alpha; neutrophil adherence receptor alpha-M subunit; integrin, alpha M (complement component 3 receptor 3 subunit); CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD_antigen: CD11b

products gene name :

ITGAM

other gene names :

ITGAM; ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6; CD11B; CR3A

uniprot entry name :

ITAM_HUMAN

reactivity :

Human

sequence length :

1152

specificity :

This assay has high sensitivity and excellent specificity for detection of human ITG/CD11b. No significant cross-reactivity or interference between human ITG/CD11b and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma and tissue homogenates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 0.195 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ITG/CD11b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ITG/CD11b present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ITG/CD11b is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ITG/CD11b bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

88501734

ncbi acc num :

NP_000623.2

ncbi gb acc num :

NM_000632.3

uniprot acc num :

P11215

ncbi mol weight :

127,179 Da

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (198795); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Hemostasis Pathway (106028)

ncbi summary :

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

uniprot summary :

Function: Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage