Rat cytochrome P450 2E1, CYP2E1 ELISA Kit | MyBioSource MBS702246 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat cytochrome P450 2E1, CYP2E1 ELISA Kit

catalog :

MBS702246

quantity :

96 Strip Wells

price :

915 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702246

products type :

ELISA Kit

products full name :

Rat cytochrome P450 2E1, CYP2E1 ELISA Kit

products short name :

cytochrome P450, family 2, subfamily E, polypeptide 1

products name syn :

Rat cytochrome P450 2E1; CYP2E1 ELISA Kit; CPE1; CYP2E; P450-J; P450C2E; cytochrome P450 2E1; cytochrome P450; subfamily IIE (ethanol-inducible); polypeptide 1; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenas; cytochrome P450; family 2; subfamily E; polypeptide 1

other names :

PREDICTED: cytochrome P450 2E1; Cytochrome P450 2E1; cytochrome P450 2E1; Cytochrome P450-ALC; Cytochrome P450 LM3A; Cytochrome P450 isozyme 3A; 4-nitrophenol 2-hydroxylase; 4-nitrophenol 2-hydroxylase (EC:1.14.13.n7); CYPIIE1; Cytochrome P450 isozyme 3A; Cytochrome P450 LM3A; Cytochrome P450-ALC

products gene name :

CYP2E1

other gene names :

LOC100342572; CYP2E1; CYP2E; CYP2E1; CYPIIE1; CYP2E; Cytochrome P450 LM3A

uniprot entry name :

CP2E1_RABIT

reactivity :

Rat

sequence length :

493

specificity :

This assay has high sensitivity and excellent specificity for detection of Rat CYP2E1. No significant cross-reactivity or interference between Rat CYP2E1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.156 ng/ml-10 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.039 ng/ml

other info2 :

Sample Volume: 50-100ul

products description :

Introduction: We are constantly exposed to many potentially toxic chemicals. Most require metabolic activation to species responsible for cell injury. Although cytochrome P450 2E1 is only one of many different forms of cytochrome P450 that catalyze these reactions, it has an important role in human health as a result of being readily induced by acute and chronic alcohol ingestion. The enzyme efficiently catalyzes the low Km metabolism of compounds commonly used as solvents in industry and at home as well as components found in cigarette smoke, many of which are established carcinogens and hepatotoxins. As a result, there is the potential for increased risk to low level exposure to such chemicals while cytochrome P450 2E1 is induced. Many substrates have been identified for cytochrome P450 2E1. Of the 52 substrates for the enzyme identified in this review, the demethylation of N,N-dimethylnitrosamine and the hydroxylation of p-nitrophenol and chlorzoxazone are the most effective for monitoring the level of this enzyme. In addition to oxidative reactions, cytochrome P450 2E1 is also an efficient catalyst of reductive reactions. CCl4-induced hepatotoxicity is one of the best- documented cases for the participation of cytochrome P450 2E1 in a toxicologically important reductive reaction. The reduction of oxygen to superoxide and peroxide are also important reductive reactions of the enzyme and could be important in lipid peroxidation. However, the role of this reaction in vivo remains controversial. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYP2E1. Standards or samples are then added to the 3 appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CYP2E1. and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CYP2E1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CYP2E1. in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

291404953

ncbi acc num :

XP_002718818.1

ncbi gb acc num :

XM_002718772.2

uniprot acc num :

P08682

ncbi mol weight :

56,945 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway 82991!!Arachidonic Acid Metabolism Pathway 366!!Biological Oxidations Pathway 105698!!CYP2E1 Reactions Pathway 105705!!Cytochrome P450 - Arranged By Substrate Type Pathway 105700!!Drug Metabolism - Cytochrome P450 Pathway 83032!!Drug Metabolism - Cytochrome P450 Pathway 427!!Fatty Acid Omega Oxidation Pathway 198915!!Linoleic Acid Metabolism Pathway 82992!!Linoleic Acid Metabolism Pathway 367

uniprot summary :

Function: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites . Catalytic activity: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. Cofactor: Heme group

size :

96 Strip Wells

price :

915 USD

more info or order :

MyBioSource product webpage