catalog number :
MBS702239
products type :
ELISA Kit
products full name :
Human Intestinal-type alkaline phosphatase, ALPI ELISA Kit
products short name :
alkaline phosphatase, ALP
products name syn :
Human alkaline phosphatase; ALP ELISA Kit; alkaline phosphatase; ALP
other names :
antileukoproteinase; Antileukoproteinase; antileukoproteinase; ALP; secretory leukocyte protease inhibitor; secretory leukocyte peptidase inhibitor; Secretory leukocyte protease inhibitor
other gene names :
Slpi; Slpi; ALP
uniprot entry name :
SLPI_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Human ALP. No significant cross-reactivity or interference between Human ALP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml-200 ng/ml. Sensitivity: 0.78 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ALP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ALP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_035544.1
ncbi gb acc num :
NM_011414.3
ncbi mol weight :
14,308 Da
uniprot summary :
Function: Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues
size4 :
10x96-Strip-Wells