Rat soluble receptor activator of nuclear factor-kB ligand, sRANKL ELISA Kit | MyBioSource MBS702223 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat soluble receptor activator of nuclear factor-kB ligand, sRANKL ELISA Kit

catalog :

MBS702223

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702223

products type :

ELISA Kit

products full name :

Rat soluble receptor activator of nuclear factor-kB ligand, sRANKL ELISA Kit

products short name :

tumor necrosis factor (ligand) superfamily, member 11

products name syn :

Rat soluble receptor activator of nuclear factor-kB ligand; sRANKL ELISA Kit; CD254; ODF; OPGL; OPTB2; RANKL; TRANCE; hRANKL2; sOdf; OTTHUMP00000178585; TNF-related activation-induced cytokine; osteoclast differentiation factor; osteoprotegerin ligand; receptor activator of nucle; tumor necrosis factor (ligand) superfamily; member 11

other names :

tumor necrosis factor ligand superfamily member 11; Tumor necrosis factor ligand superfamily member 11; tumor necrosis factor ligand superfamily member 11; ODF; OPGL; TRANCE; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; tumor necrosis factor (ligand) superfamily, member 11; Osteoclast differentiation factor; ODF; Osteoprotegerin ligand; OPGL; Receptor activator of nuclear factor kappa-B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE; CD_antigen: CD254Cleaved into the following 2 chains:Tumor necrosis factor ligand superfamily member 11, membrane form; Tumor necrosis factor ligand superfamily member 11, soluble form

products gene name :

TNFSF11

other gene names :

Tnfsf11; Tnfsf11; RANKL; Opgl; Rankl; Trance; ODF; OPGL; RANKL; TRANCE

uniprot entry name :

TNF11_RAT

reactivity :

Rat

sequence length :

318

specificity :

This assay has high sensitivity and excellent specificity for detection of Rat TNFSF11. No significant cross-reactivity or interference between Rat TNFSF11 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: 15.6 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Differentiation

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sRANKL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sRANKL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sRANKL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sRANKL bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

16924012

ncbi acc num :

NP_476490.1

ncbi gb acc num :

NM_057149.1

uniprot acc num :

Q9ESE2

ncbi mol weight :

35,370 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); NF-kappa B Signaling Pathway (634541); Osteoblast Pathway (198455); Osteoclast Pathway (198474); Osteoclast Differentiation Pathway (193140); Osteoclast Differentiation Pathway (193096); Prolactin Signaling Pathway (814205); Prolactin Signaling Pathway (814248); Rheumatoid Arthritis Pathway (200306)

ncbi summary :

induces osteoclast formation and bone resoprtion; plays a role in regulation of calcium homeostasis and osteoclast function [RGD, Feb 2006]

uniprot summary :

Function: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage