ELISA/assay

Dog interleukin 4, IL-4 ELISA Kit

MBS702215

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Dog interleukin 4, IL-4 ELISA Kit

[interleukin 4]

[Dog interleukin 4; IL-4 ELISA Kit; BCGF-1; BCGF1; BSF1; IL-4; MGC79402; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; interleukin 4]

[interleukin-4; Interleukin-4; interleukin-4; BSF-1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell stimulatory factor 1; BSF-1; Lymphocyte stimulatory factor 1]

[IL4]

[IL4; IL4; IL-4; IL-4; BSF-1]

IL4_CANFA

Dog

132

This assay has high sensitivity and excellent specificity for detection of dog IL-4. No significant cross-reactivity or interference between dog IL-4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml-400 pg/ml. Sensitivity: Typically less than 1.56 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

50978886

NP_001003159.1

NM_001003159.1

O77762

15,267 Da

Allograft Rejection Pathway (84169); Allograft Rejection Pathway (535); Asthma Pathway (84166); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (84167); Autoimmune Thyroid Disease Pathway (533); Cytokine-cytokine Receptor Interaction Pathway (84101); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198591); Fc Epsilon RI Signaling Pathway (84132)

Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

450

96-Strip-Wells

640

5x96-Strip-Wells

2615

10x96-Strip-Wells

4940