catalog number :
MBS702200
products type :
ELISA Kit
products full name :
Mouse Brain derived neurotrophic facor, BDNF ELISA Kit
products short name :
brain-derived neurotrophic factor
products name syn :
Mouse Brain derived neurotrophic facor; BDNF ELISA Kit; MGC34632; neurotrophin; brain-derived neurotrophic factor
other names :
brain-derived neurotrophic factor isoform 2; Brain-derived neurotrophic factor; brain-derived neurotrophic factor; anorexia BDNF; brain derived neurotrophic factor
products gene name :
BDNF
other gene names :
Bdnf; Bdnf; BDNF
uniprot entry name :
BDNF_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse BDNF. No significant cross-reactivity or interference between mouse BDNF and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 3.9 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BDNF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BDNF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BDNF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BDNF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001041604.1
ncbi gb acc num :
NM_001048139.1
ncbi mol weight :
28,123 Da
ncbi pathways :
Alcoholism Pathway (585577); Alcoholism Pathway (587116); Cocaine Addiction Pathway (546267); Cocaine Addiction Pathway (546273); Huntington's Disease Pathway (83297); Huntington's Disease Pathway (512); MAPK Signaling Pathway (198294); MAPK Signaling Pathway (83245); MAPK Signaling Pathway (456); Neurotrophin Signaling Pathway (101156)
ncbi summary :
The protein encoded by this gene is a member of the nerve growth factor family. It is involved in the growth, differentiation and survival of specific types of developing neurons both in the central nervous system (CNS) and the peripheral nervous system. It is also involved in regulating synaptic plasticity in the CNS. Expression of a similar gene in human is reduced in both Alzheimer's and Huntington disease patients. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Sep 2015]
uniprot summary :
Function: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS.
size4 :
10x96-Strip-Wells