catalog number :
MBS702191
products type :
ELISA Kit
products full name :
Mouse antidiuretic hormone/vasopressin/arginine vasopressin, ADH/VP/AVP ELISA Kit
products short name :
arginine vasopressin
products name syn :
Mouse antidiuretic hormone/vasopressin/arginine vasopressin; ADH/VP/AVP ELISA Kit; ADH; ARVP; AVP-NPII; AVRP; VP; antidiuretic hormone; arginine vasopressin-neurophysin II; neurohypophyseal; vasopressin-neurophysin II-copeptin; arginine vasopressin
other names :
vasopressin-neurophysin 2-copeptin preproprotein; Vasopressin-neurophysin 2-copeptin; vasopressin-neurophysin 2-copeptin; AVP-NPII; arginine vasopressin; AVP-NPIICleaved into the following 3 chains:Arg-vasopressinAlternative name(s):Arginine-vasopressin
other gene names :
Avp; Avp; Vp; Vsp
uniprot entry name :
NEU2_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse AVP. No significant cross-reactivity or interference between Mouse AVP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates. Detection Range: 1.25 pg/ml-80 pg/ml. Sensitivity: 0.31 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ADH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ADH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ADH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_033862.1
ncbi gb acc num :
NM_009732.1
ncbi mol weight :
17,900 Da
ncbi pathways :
Aquaporin-mediated Transport Pathway (1001362); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (1000462); Bmal1:Clock,Npas2 Activates Circadian Gene Expression Pathway (966898); Circadian Clock Pathway (966897); Circadian Clock Pathway (1000461); Class A/1 (Rhodopsin-like Receptors) Pathway (1000942); G Alpha (q) Signalling Events Pathway (1000975); G Alpha (s) Signalling Events Pathway (1000972); GPCR Downstream Signaling Pathway (1000971); GPCR Ligand Binding Pathway (1000941)
ncbi summary :
This gene encodes a member of the vasopressin/oxytocin family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the neuropeptide hormone arginine vasopressin, and two other peptides, neurophysin 2 and copeptin. Arginine vasopressin binds to vasopressin receptors and functions as a vasopressor, to constrict blood vessels and increase blood pressure, and as an antidiuretic, to reduce the production of urine. Neurophysin 2 functions as a carrier protein in the transport of arginine vasopressin. This gene is present in a gene cluster with the related gene oxytocin on chromosome 2. [provided by RefSeq, Aug 2015]
uniprot summary :
Function: Neurophysin 2 specifically binds vasopressin.
size4 :
10x96-Strip-Wells