ELISA/assay

Human Azurocidin/Heparin Binding Protein, AZU/HBP ELISA Kit

MBS702182

96 Strip Wells

850 USD

ELISA Kit

Human Azurocidin/Heparin Binding Protein, AZU/HBP ELISA Kit

azurocidin 1

Human Azurocidin/Heparin Binding Protein (AZU/HBP) ELISA Kit; AZAMP; AZU; CAP37; HBP; HUMAZUR; NAZC; cationic antimicrobial protein 37; heparin-binding protein; neutrophil azurocidin; azurocidin 1

azurocidin preproprotein; Azurocidin; azurocidin; neutrophil azurocidin; heparin-binding protein; cationic antimicrobial protein 37; cationic antimicrobial protein CAP37; azurocidin 1; Cationic antimicrobial protein CAP37; Heparin-binding protein; HBP

AZU1

AZU1; AZU1; AZU; HBP; NAZC; AZAMP; CAP37; HUMAZUR; HBP

CAP7_HUMAN

Human

251

This assay has high sensitivity and excellent specificity for detection of Human AZU1. No significant cross-reactivity or interference between Human AZU1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, urine, tissue homogenates, cell culture supernates. Detection Range: 7.8pg/ml-500pg/ml. Detection Wavelength: 450 nm. Sensitivity: 1.95 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 2: chemokine. Protein Biological Process 3: Chemotaxis

Introduction: Azurocidin is a protein that in humans is encoded by the AZU1 gene. Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. The protein encoded by this gene is an azurophil granule antibiotic protein, with monocyte chemotactic and antibacterial activity. It is also an important multifunctional inflammatory mediator. This encoded protein is a member of the serine protease gene family but it is not a serine proteinase, because the active site serine and histidine residues are replaced. The genes encoding this protein, neutrophil elastase 2, and proteinase 3 are in a cluster located at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AZU. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for AZU and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain AZU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AZU in the samples is then determined by comparing the O.D. of the samples to the standard curve.

11342670

NP_001691.1

NM_001700.3

P20160

26,886 Da

Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. The protein encoded by this gene is an azurophil granule antibiotic protein, with monocyte chemotactic and antibacterial activity. It is also an important multifunctional inflammatory mediator. This encoded protein is a member of the serine protease gene family but it is not a serine proteinase, because the active site serine and histidine residues are replaced. The genes encoding this protein, neutrophil elastase 2, and proteinase 3 are in a cluster located at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, Jul 2008]

Function: This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.2 PublicationsManual assertion based on experiment in:Ref.10

96 Strip Wells

850 USD