ELISA/assay

Human Vascular Endothelial cell Growth Factor B, VEGF-B ELISA Kit

MBS702175

48-Strip-Wells

435 USD

ELISA Kit

Human Vascular Endothelial cell Growth Factor B, VEGF-B ELISA Kit

vascular endothelial growth factor B

Human Vascular Endothelial cell Growth Factor B; VEGF-B ELISA KIT; VEGFL; VRF; ; vascular endothelial growth factor B

vascular endothelial growth factor B isoform VEGFB-167; Vascular endothelial growth factor B; vascular endothelial growth factor B; VEGF-related factor; vascular endothelial growth factor B; VEGF-related factor; VRF

VEGFB

VEGFB; VEGFB; VRF; VEGFL; VRF; VEGF-B; VRF

VEGFB_HUMAN

Human

188

This assay has high sensitivity and excellent specificity for detection of Human VEGFB. No significant cross-reactivity or interference between Human VEGFB and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGF-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF-B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF-B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF-B bound in the initial step. The color development is stopped and the intensity of the color is measured.

344179103

NP_001230662.1

NM_001243733.1

P49765

21,602 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Heart Development Pathway (198802); Hemostasis Pathway (106028); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Pathways In Cancer (83105)

This gene encodes a member of the PDGF (platelet-derived growth factor)/VEGF (vascular endothelial growth factor) family. The VEGF family members regulate the formation of blood vessels and are involved in endothelial cell physiology. This member is a ligand for VEGFR-1 (vascular endothelial growth factor receptor 1) and NRP-1 (neuropilin-1). Studies in mice showed that this gene was co-expressed with nuclear-encoded mitochondrial genes and the encoded protein specifically controlled endothelial uptake of fatty acids. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Sep 2011]

Function: Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.

48-Strip-Wells

435 USD

96-Strip-Wells

615

5x96-Strip-Wells

2340

10x96-Strip-Wells

4370