ELISA/assay

Rat heat shock protein 70, hSP-70 ELISA Kit

MBS702173

96 Strip Wells

915 USD

ELISA Kit

Rat heat shock protein 70, hSP-70 ELISA Kit

heat shock 70kDa protein 1A

Rat heat shock protein 70; hSP-70 ELISA Kit; DAQB-147D11.1; FLJ54303; FLJ54370; FLJ54392; FLJ54408; FLJ75127; HSP70-1; HSP70-1A; HSP70I; HSP72; HSPA1; HSPA1B; dnaK-type molecular chaperone HSP70-1; heat shock 70kD protein 1A; heat shock-induced; heat shock 70kDa protein 1A

heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1A/1B; heat shock 70 kDa protein 1A/1B; HSP70.1/2; HSP70-1/HSP70-2; heat shock protein 70-1; heat shock 70 kDa protein 1/2; heat shock 70kD protein 1A; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2

HSPA1A

Hspa1a; Hspa1a; HSP72; Hspa1; Hspa1b; Hsp70-1; Hsp70-1; Hspa1 Hspa1b ; Hsp70-2; Hspa2; HSP70-1/HSP70-2; HSP70.1/HSP70.2

HSP71_RAT

Rat

641

This assay has high sensitivity and excellent specificity for detection of Rat HSPA1A. No significant cross-reactivity or interference between Rat HSPA1A and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 62.5 pg/ml-4000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 15.6 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Neurobiology. Protein Biological Process 3: Stress response

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for hSP-70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any hSP-70 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for hSP-70 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of hSP-70 bound in the initial step. The color development is stopped and the intensity of the color is measured.

260064045

NP_114177.2

NM_031971.2

Q07439

70,185 Da

AUF1 (hnRNP D0) Destabilizes MRNA Pathway 1010471!!Androgen Receptor Signaling Pathway 198529!!Antigen Processing And Presentation Pathway 83466!!Antigen Processing And Presentation Pathway 485!!Apoptosis Modulation By HSP70 Pathway 198484!!Attenuation Phase Pathway 1010384!!Cardiovascular Signaling Pathway 219769!!Cellular Response To Heat Stress Pathway 1010381!!Cellular Responses To Stress Pathway 1010374!!Endocytosis Pathway 102267

heat inducible gene; induced by global ischemia and kainic acid-induced seizures [RGD, Feb 2006]

Function: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage

96 Strip Wells

915 USD