catalog number :
MBS702170
products type :
ELISA Kit
products full name :
Mouse Gelsolin ELISA Kit
products short name :
gelsolin (amyloidosis, Finnish type)
products name syn :
Mouse Gelsolin ELISA Kit; RP11-477J21.1; DKFZp313L0718; OTTHUMP00000162202; gelsolin; gelsolin (amyloidosis; Finnish type)
other names :
gelsolin isoform 1; Gelsolin; gelsolin; brevin; actin-depolymerizing factor; gelsolin; Actin-depolymerizing factor; ADF; Brevin
other gene names :
Gsn; Gsn; ADF; Gsb; ADF
uniprot entry name :
GELS_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse GSN. No significant cross-reactivity or interference between Mouse GSN and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 15.6 ng/ml-1000 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 3.9 ng/ml
other info2 :
Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Amino-acid biosynthesis and metabolism. Protein Biological Process 3: Cilium biogenesis/degradation
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Gelsolin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Gelsolin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Gelsolin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Gelsolin bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_666232.2
ncbi gb acc num :
NM_146120.4
ncbi mol weight :
85,942 Da
ncbi pathways :
Amyloids Pathway 1000612!!Apoptosis Pathway 970512!!Apoptotic Cleavage Of Cellular Proteins Pathway 1000450!!Apoptotic Execution Phase Pathway 1000449!!Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway 1000451!!Disease Pathway 970606!!Fc Gamma R-mediated Phagocytosis Pathway 114297!!Fc Gamma R-mediated Phagocytosis Pathway 106590!!Regulation Of Actin Cytoskeleton Pathway 198389!!Regulation Of Actin Cytoskeleton Pathway 83286
uniprot summary :
Function: Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.