ELISA/assay

Rat Interleukin 16, IL-16 ELISA Kit

MBS702167

48-Strip-Wells

395 USD

ELISA Kit

Rat Interleukin 16, IL-16 ELISA Kit

Interleukin 16, IL-16

Rat Interleukin 16; IL-16 ELISA KIT; Interleukin 16; IL-16

IL16 protein, partial; IL16 protein; pro-interleukin-16; prointerleukin 16; neuronal interleukin 16; lymphocyte chemoattractant factor; interleukin 16; IL16 protein

IL-16

IL16; IL16; LCF; NIL16; PRIL16; prIL-16

Q05BE6_HUMAN

Rat

576

This assay has high sensitivity and excellent specificity for detection of rat IL-16. No significant cross-reactivity or interference between rat IL-16 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of rat IL-16 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-16 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-16 bound in the initial step. The color development is stopped and the intensity of the color is measured.

116283877

AAH50362.1

Q05BE6

61,007 Da

The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

IL16: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. 3 isoforms of the human protein are produced by alternative promoter. Protein type: DNA-binding; Motility/polarity/chemotaxis; Cytokine. Chromosomal Location of Human Ortholog: 15q26.3. Cellular Component: extracellular space; cytoplasm; plasma membrane; nucleus. Molecular Function: cytokine activity. Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; viral reproduction; leukocyte chemotaxis; immune response; induction of positive chemotaxis

48-Strip-Wells

395 USD

96-Strip-Wells

550

5x96-Strip-Wells

2145

10x96-Strip-Wells

3995