Human complement factor I, CFI ELISA Kit | MyBioSource MBS702160 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human complement factor I, CFI ELISA Kit

catalog :

MBS702160

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702160

products type :

ELISA Kit

products full name :

Human complement factor I, CFI ELISA Kit

products short name :

complement factor I

products name syn :

Human complement factor I (CFI) ELISA Kit; AHUS3; C3BINA; C3b-INA; FI; IF; KAF; C3B/C4B inactivator; C3b-inactivator; Konglutinogen-activating factor; complement component I; complement control protein factor I; complement factor I heavy chain; li; complement factor I

other names :

complement factor I preproprotein; Complement factor I; complement factor I; C3b-inactivator; C3B/C4B inactivator; complement component I; light chain of factor I; Konglutinogen-activating factor; complement factor I heavy chain; complement control protein factor I; complement factor I; C3B/C4B inactivatorCleaved into the following 2 chains:Complement factor I heavy chain; Complement factor I light chain

products gene name :

CFI

other gene names :

CFI; CFI; FI; IF; KAF; AHUS3; ARMD13; C3BINA; C3b-INA; IF

uniprot entry name :

CFAI_HUMAN

reactivity :

Human

sequence length :

583

specificity :

This assay has high sensitivity and excellent specificity for detection of Human CFI. No significant cross-reactivity or interference between Human CFI and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Detection Range: 6.25 ng/ml-400 ng/ml. Sensitivity: 1.56 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Complement. Protein Biological Process 3: Complement pathway

products description :

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CFI. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CFI and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CFI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CFI in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

119392081

ncbi acc num :

NP_000195.2

ncbi gb acc num :

NM_000204.3

uniprot acc num :

P05156

ncbi mol weight :

65,750 Da

ncbi pathways :

Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Immune System Pathway (106386); Innate Immune System Pathway (106387); Regulation Of Complement Cascade Pathway (576254); Staphylococcus Aureus Infection Pathway (172846); Staphylococcus Aureus Infection Pathway (171867)

ncbi summary :

This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uraemic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immmune deposits is another condition associated with mutation of this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Responsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively. Catalytic activity: Inactivates complement subcomponents C3b, iC3b and C4b by proteolytic cleavage.

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage