ELISA/assay

Human Pulmonary surfactant-associated protein C, SP-C ELISA Kit

MBS702157

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human Pulmonary surfactant-associated protein C, SP-C ELISA Kit

[surfactant protein C]

[Human Pulmonary surfactant-associated protein C; SP-C ELISA Kit; PSP-C; SFTP2; SMDP2; SP-C; pulmonary surfactant apoprotein-2 SP-C; surfactant; pulmonary-associated protein C; surfactant protein C]

[pulmonary surfactant-associated protein C isoform 2 proprotein; Pulmonary surfactant-associated protein C; pulmonary surfactant-associated protein C; SP5; BRICHOS domain containing 6; pulmonary surfactant apoprotein-2 SP-C; pulmonary surfactant-associated proteolipid SPL(Val); surfactant protein C; Pulmonary surfactant-associated proteolipid SPL(Val); SP5]

[SFTPC]

[SFTPC; SFTPC; SP-C; PSP-C; SFTP2; SMDP2; BRICD6; SFTP2; SP-C]

PSPC_HUMAN

Human

191

This assay has high sensitivity and excellent specificity for detection of bovine SP-C. No significant cross-reactivity or interference between bovine SP-C and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 12.5 ng/ml-800 ng/ml. Sensitivity: 3.12 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP-C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP-C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-C is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-C bound in the initial step. The color development is stopped and the intensity of the color is measured.

288915543

NP_001165828.1

NM_001172357.1

P11686

21,053 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified.[provided by RefSeq, Feb 2010]

Function: Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145