ELISA/assay

Human lysosomal-associated membrane protein 2, HLAMP-2 ELISA Kit

MBS702152

48-Strip-Wells

565 USD

ELISA Kit

Human lysosomal-associated membrane protein 2, HLAMP-2 ELISA Kit

lysosomal-associated membrane protein 2, HLAMP-2

Human lysosomal-associated membrane protein 2; HLAMP-2 ELISA Kit; CD107b; LAMPB; LGP110; lysosome-associated membrane protein 2; lysosomal-associated membrane protein 2; HLAMP-2

lysosome-associated membrane glycoprotein 2 isoform C; Lysosome-associated membrane glycoprotein 2; lysosome-associated membrane glycoprotein 2; CD107 antigen-like family member B; lysosomal-associated membrane protein 2; CD107 antigen-like family member B; CD_antigen: CD107b

HLAMP-2

LAMP2; LAMP2; LAMPB; CD107b; LAMP-2; LGP110; LAMP-2; Lysosome-associated membrane protein 2

LAMP2_HUMAN

Human

411

This assay has high sensitivity and excellent specificity for detection of Human HLAMP-2. No significant cross-reactivity or interference between Human HLAMP-2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HLAMP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HLAMP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HLAMP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HLAMP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

169790833

NP_001116078.1

NM_001122606.1

P13473

44,961 Da

Hemostasis Pathway (106028); Lysosome Pathway (99052); Lysosome Pathway (96865); Phagosome Pathway (153910); Phagosome Pathway (153859); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048); Senescence And Autophagy Pathway (198780); Tuberculosis Pathway (213780)

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008]

Function: Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.1 PublicationManual assertion based on experiment in:Ref.3

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150