ELISA/assay

Rat P-Selectin ELISA Kit

MBS702140

48-Strip-Wells

465 USD

ELISA Kit

Rat P-Selectin ELISA Kit

selectin P (granule membrane protein 140kDa, antigen CD62)

Rat P-Selectin ELISA kit; RP1-86F14.2; CD62; CD62P; FLJ45155; GMP140; GRMP; LECAM3; PADGEM; PSEL; OTTHUMP00000032609; granulocyte membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet alpha-granule membrane; selectin P (granule membrane protein 140kDa; antigen CD62)

P-selectin; P-selectin; P-selectin; LECAM3; PADGEM; GMP-140; selectin, platelet; granule membrane protein 140; CD62 antigen-like family member P; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein; selectin P; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD_antigen: CD62P

SELP

Selp; Selp; PSELECT; GMP-140; LECAM3; PADGEM

LYAM3_RAT

Rat

768

This assay has high sensitivity and excellent specificity for detection of Rat SELP. No significant cross-reactivity or interference between Rat SELP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Detection Range: 9.38 ng/ml–600 ng/ml. Sensitivity: 2.34ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cell Adhesion. Protein Biological Process 3: Cell adhesion

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to P-Selectin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for P-Selectin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain P-Selectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of P-Selectin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

6981524

NP_037246.1

NM_013114.1

P98106

83,517 Da

Cell Adhesion Molecules (CAMs) Pathway (83461); Cell Adhesion Molecules (CAMs) Pathway (480); IL-3 Signaling Pathway (198504); Malaria Pathway (152662); Malaria Pathway (152657); Spinal Cord Injury Pathway (739006); Staphylococcus Aureus Infection Pathway (172815); Staphylococcus Aureus Infection Pathway (171867)

mediates leukocyte interaction with platelets and endothelial binding after vascular injury; may play a role in inflammatory response [RGD, Feb 2006]

Function: Ca2+-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1

48-Strip-Wells

465 USD

96-Strip-Wells

655

5x96-Strip-Wells

2455

10x96-Strip-Wells

4595